fix lyse solution (Thermo Fisher)
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fix lyse solution
Fix Lyse Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fix+lyse+solution/Phosphate/pm41400336-57-8-10
Average 99 stars, based on 1 article reviews
Fix Lyse Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fix+lyse+solution/Phosphate/pm41400336-57-8-10
Average 99 stars, based on 1 article reviews
fix lyse solution - by Bioz Stars,
2026-10
99/100 stars
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Blocking Assay:Article Title: Phenotypic Characterization and Isolation of Myeloid-Derived Suppressor Cells. Article Snippet: .. Antibodies: Anti-human leukocyte antigen DR isotype (HLA-DR) Pacific Blue, clone LN3 (e.g., BioLegend, cat. no. 327016) Anti-human CD33 PE, clone WM53 (e.g., BioLegend, cat. no. 303404) Anti-human CD14 PE/Cy7, clone 63D3 (e.g., BioLegend, cat. no. 367112) Anti-human CD15 FITC, clone W6D3 (e.g., BioLegend, cat. no. 323003) Anti-human CD11b APC, clone ICRF44 (e.g., BioLegend, cat. no. 301350) Human TruStain FcX, Fc receptor blocking solution (e.g., BioLegend, cat. no. 422302) FACS buffer (see recipe) Human whole blood sample (see Basic Protocol 5) 10× 1-Step FACS:Article Title: Phenotypic Characterization and Isolation of Myeloid-Derived Suppressor Cells. Article Snippet: .. 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Antibodies: Anti-human leukocyte antigen DR isotype (HLA-DR) Pacific Blue, clone LN3 (e.g., BioLegend, cat. no. 327016) Anti-human CD33 PE, clone WM53 (e.g., BioLegend, cat. no. 303404) Anti-human CD14 PE/Cy7, clone 63D3 (e.g., BioLegend, cat. no. 367112) Anti-human CD15 FITC, clone W6D3 (e.g., BioLegend, cat. no. 323003) Anti-human CD11b APC, clone ICRF44 (e.g., BioLegend, cat. no. 301350) Human TruStain FcX, Fc receptor blocking solution (e.g., BioLegend, cat. no. 422302) FACS buffer (see recipe) Human whole blood sample (see Basic Protocol 5) 10× 1-Step Article Title: Long-acting lenacapavir protects macaques against intravenous challenge with simian-tropic HIV Article Snippet: For the assay, 50 μL EDTA–anti-coagulated whole blood was incubated for 20 min with 50 μL of the following titrated and optimized antibodies: CD20 Pacific Blue (clone 2H7; RRID: AB_493651), CD28 Brilliant Violet (BV) 510 (clone CD28.2; RRID: AB_2562030), CD45 Brilliant Ultra Violet (BUV) 805 (clone DO58-1283, BD; RRID: AB_2871344), CD16 BV785 (clone 3G8; RRID: AB_2563803), CD123 PE-Dazzle 594 (clone 6H6; RRID: AB_2566450), CD11c APC (clone S-HCL-3; RRID: AB_2616902), CD137 BV650 (clone 4B4-1; RRID: AB_2572193), CD56 BUV615 (clone B159, BD; RRID: AB_2875357), HLA-DR FITC (clone L243; RRID: AB_1089142), CD8b BUV496 (clone 2ST8.5H7, BD; AB_2874080), CD3 PE (clone SP34-2, BD; AB_394342), CD95 PE-Cy5 (clone DX2; RRID: AB_314548), CD8α PE-Cy7 (clone SK1; RRID: AB_2044008), CD4 BV711 (clone OKT4; RRID: AB_2562912), and CD14 APC-Fire 750 (clone M5E2; RRID: AB_493695). .. 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Antibodies: Anti-human leukocyte antigen DR isotype (HLA-DR) Pacific Blue, clone LN3 (e.g., BioLegend, cat. no. 327016) Anti-human CD33 PE, clone WM53 (e.g., BioLegend, cat. no. 303404) Anti-human CD14 PE/Cy7, clone 63D3 (e.g., BioLegend, cat. no. 367112) Anti-human CD15 FITC, clone W6D3 (e.g., BioLegend, cat. no. 323003) Anti-human CD11b APC, clone ICRF44 (e.g., BioLegend, cat. no. 301350) Human TruStain FcX, Fc receptor blocking solution (e.g., BioLegend, cat. no. 422302) FACS buffer (see recipe) Human whole blood sample (see Basic Protocol 5) 10× 1-Step Article Title: Transfusion of adult platelets triggers inflammatory responses in newborn mice through both P-selectin-dependent and -independent mechanisms. Article Snippet: Funding information National Heart, Lung, and Blood Institute, Grant/Award Numbers: P01-HL046925, K99 HL156051-01 Abstract Background: Liberal platelet transfusion practices increase neonatal morbidity and mortality.. 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Murine blood was stained the same way as described above, but after staining, samples were incubated with eBioscience 1 Step Article Title: Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples Article Snippet: For the pilot study, cryopreserved PBMC samples were thawed, up to 1 × 106 cells were stained with 50 μl of an antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences) and -CD14 (M5E2, BD biosciences) plus Zombie-Near IR live/dead (Biolegend) and data were collected on a BD Biosciences LSRFortessaTM X-20. .. For analysis of whole blood samples stored at RT, 1 μl of undiluted Zombie NIR stain was added to 100 μl whole blood and incubated in the dark for 10 min. Fifty microlitres of antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences), -CD8 (HIT8a, BD biosciences), -CD19 (HIB19, BD biosciences), -CD56 (NCAM16.2, BD biosciences), -CD14 (M5E2, Biolegend) and -CD16 (3G8, BD biosciences) was added and incubated at RT for 30 min. Two millilitres of Article Title: Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples Article Snippet: For the pilot study, cryopreserved PBMC samples were thawed, up to 1 × 10 6 cells were stained with 50 μl of an antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences) and -CD14 (M5E2, BD biosciences) plus Zombie-Near IR live/dead (Biolegend) and data were collected on a BD Biosciences LSRFortessaTM X-20. .. 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Murine blood was stained the same way as described above, but after staining, samples were incubated with eBioscience 1 Step Article Title: Long-acting lenacapavir protects macaques against intravenous challenge with simian-tropic HIV Article Snippet: For the assay, 50 μL EDTA–anti-coagulated whole blood was incubated for 20 min with 50 μL of the following titrated and optimized antibodies: CD20 Pacific Blue (clone 2H7; RRID: AB_493651), CD28 Brilliant Violet (BV) 510 (clone CD28.2; RRID: AB_2562030), CD45 Brilliant Ultra Violet (BUV) 805 (clone DO58-1283, BD; RRID: AB_2871344), CD16 BV785 (clone 3G8; RRID: AB_2563803), CD123 PE-Dazzle 594 (clone 6H6; RRID: AB_2566450), CD11c APC (clone S-HCL-3; RRID: AB_2616902), CD137 BV650 (clone 4B4-1; RRID: AB_2572193), CD56 BUV615 (clone B159, BD; RRID: AB_2875357), HLA-DR FITC (clone L243; RRID: AB_1089142), CD8b BUV496 (clone 2ST8.5H7, BD; AB_2874080), CD3 PE (clone SP34-2, BD; AB_394342), CD95 PE-Cy5 (clone DX2; RRID: AB_314548), CD8α PE-Cy7 (clone SK1; RRID: AB_2044008), CD4 BV711 (clone OKT4; RRID: AB_2562912), and CD14 APC-Fire 750 (clone M5E2; RRID: AB_493695). .. Samples were then incubated for 10 min with 2 ml of 1X 1-Step Article Title: Linker Histone H1 subtypes specifically regulate neutrophil differentiation Article Snippet: .. Murine blood was stained the same way as described above, but after staining, samples were incubated with eBioscience 1 Step Article Title: Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples Article Snippet: For the pilot study, cryopreserved PBMC samples were thawed, up to 1 × 106 cells were stained with 50 μl of an antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences) and -CD14 (M5E2, BD biosciences) plus Zombie-Near IR live/dead (Biolegend) and data were collected on a BD Biosciences LSRFortessaTM X-20. .. For analysis of whole blood samples stored at RT, 1 μl of undiluted Zombie NIR stain was added to 100 μl whole blood and incubated in the dark for 10 min. Fifty microlitres of antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences), -CD8 (HIT8a, BD biosciences), -CD19 (HIB19, BD biosciences), -CD56 (NCAM16.2, BD biosciences), -CD14 (M5E2, Biolegend) and -CD16 (3G8, BD biosciences) was added and incubated at RT for 30 min. Two millilitres of Article Title: Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples Article Snippet: For the pilot study, cryopreserved PBMC samples were thawed, up to 1 × 10 6 cells were stained with 50 μl of an antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences) and -CD14 (M5E2, BD biosciences) plus Zombie-Near IR live/dead (Biolegend) and data were collected on a BD Biosciences LSRFortessaTM X-20. .. For analysis of whole blood samples stored at RT, 1 μl of undiluted Zombie NIR stain was added to 100 μl whole blood and incubated in the dark for 10 min. Fifty microlitres of antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences), -CD8 (HIT8a, BD biosciences), -CD19 (HIB19, BD biosciences), -CD56 (NCAM16.2, BD biosciences), -CD14 (M5E2, Biolegend) and -CD16 (3G8, BD biosciences) was added and incubated at RT for 30 min. Two millilitres of Saline:Article Title: Transfusion of adult platelets triggers inflammatory responses in newborn mice through both P-selectin-dependent and -independent mechanisms. Article Snippet: Funding information National Heart, Lung, and Blood Institute, Grant/Award Numbers: P01-HL046925, K99 HL156051-01 Abstract Background: Liberal platelet transfusion practices increase neonatal morbidity and mortality.. The mechanisms underlying this harm are unknown but may involve the immune rather than hemostatic functions of platelets, as well as the significant differences between adult (transfused) and neonatal platelets, particularly the higher P-selectin surface expression on activated adult platelets.. In this study, we investigated the immune/inflammatory effects of transfusing adult platelets into newborn mice. Sterility:Article Title: The mechanical arthropod vector Stomoxys calcitrans influences the outcome of lumpy skin disease virus infection in cattle Article Snippet: .. 2 mL eBioscience™ Recombinase Polymerase Amplification:Article Title: Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples Article Snippet: For the pilot study, cryopreserved PBMC samples were thawed, up to 1 × 106 cells were stained with 50 μl of an antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences) and -CD14 (M5E2, BD biosciences) plus Zombie-Near IR live/dead (Biolegend) and data were collected on a BD Biosciences LSRFortessaTM X-20. .. For analysis of whole blood samples stored at RT, 1 μl of undiluted Zombie NIR stain was added to 100 μl whole blood and incubated in the dark for 10 min. Fifty microlitres of antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences), -CD8 (HIT8a, BD biosciences), -CD19 (HIB19, BD biosciences), -CD56 (NCAM16.2, BD biosciences), -CD14 (M5E2, Biolegend) and -CD16 (3G8, BD biosciences) was added and incubated at RT for 30 min. Two millilitres of Article Title: Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples Article Snippet: For the pilot study, cryopreserved PBMC samples were thawed, up to 1 × 10 6 cells were stained with 50 μl of an antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences) and -CD14 (M5E2, BD biosciences) plus Zombie-Near IR live/dead (Biolegend) and data were collected on a BD Biosciences LSRFortessaTM X-20. .. For analysis of whole blood samples stored at RT, 1 μl of undiluted Zombie NIR stain was added to 100 μl whole blood and incubated in the dark for 10 min. Fifty microlitres of antibody cocktail comprising anti-CD3 (clone UCHT1, BD biosciences), -CD4 (RPA-T4, BD biosciences), -CD8 (HIT8a, BD biosciences), -CD19 (HIB19, BD biosciences), -CD56 (NCAM16.2, BD biosciences), -CD14 (M5E2, Biolegend) and -CD16 (3G8, BD biosciences) was added and incubated at RT for 30 min. Two millilitres of |
